How to explain this result got from DEXSeq?

[xmubingo]

Hi everyone,

it's really a helpful tool!

I got a result from DEXseq in two conditions, "H" vs. "A". but i do not how to explain it.

1. My first question is "why E002, E003, E012, E014 and E015 are differentially expressed exons? " I think they are not, because expression value in exon2 under condition 'A' is very close to value under condition 'H'. Also exon3 and exon14.

2. "if E002 is a differentially expressed exon, why E001 isn't?" because E001 is similar to E002 in the figure. They have almost same expression values under two conditions.

Thanks a lot!

[dpryan]

We'd have to see the variance in each exon to have a clue whether that's reasonable or not. We would also need to see the experimental design to see what variances are being fit.