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  • lavik17
    Member
    • Nov 2013
    • 10

    #1

    Velvet de-novo in Geneious - read length error

    Hi everyone,
    I'm trying to use Velvet plug-in for Geneious v7 in order to create contigs from metagenomic short reads (90-200bp, 100k reads).

    I get this error:
    Assemblies in the Short Read category can handle a maximum of 2 different read lengths. Consider trimming variable length read data to a uniform read length.
    Do I have to trim all reads to the same length ? It doesn't make sense, as I will lose ~20k reads which are less than 150bp, as well as all the bp in the reads which are longer than 150bp.

    Is there a better way to do it ?
  • lavik17
    Member
    • Nov 2013
    • 10

    #2
    bumping....
    Does anyone have any experience with the Velvet plug-in for Geneious ?

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