Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • Ehicares 2023
    Junior Member
    • Apr 2023
    • 3

    #1

    FASTQC report

    having a failed Per sequence GC content after running fastqc and this persisted after trimming using trimmomatic
    Click image for larger version

Name:	image.png
Views:	279
Size:	173.6 KB
ID:	324313​what else can be done to correct this. No overrepresented sequence

    ​
  • GenomicSeq
    Member
    • Oct 2022
    • 39

    #2
    Hello Ehicares 2023

    What are the quality of your reads? And what were you running (application, library prep, and sequencer used)?

    Also, what is the coverage like? And have you tried another tool to check the GC content?

    Comment

    Latest Articles

    Collapse

    • kinpict.com
      Reply to 16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
      by kinpict.com
      The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.
      Today, 06:37 PM
    • SEQadmin2
      16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
      by SEQadmin2





      NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
      10-30-2026, 06:46 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 11-12-2026, 07:59 AM
    0 responses
    26 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 11-05-2026, 09:25 AM
    0 responses
    35 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 11-03-2026, 11:54 AM
    2 responses
    55 views
    0 reactions
    Last Post kinpict.com  
    Started by SEQadmin2, 10-28-2026, 09:57 AM
    0 responses
    175 views
    0 reactions
    Last Post SEQadmin2  
    Working...