Tweet
Hello,
I'm wondering how one can I know if a gene has a germline or somatic mutation from the data I have.
-
Hello,
I'm wondering how one can I know if a gene has a germline or somatic mutation from the data I have.
-
Hi BAT - With the data just as you have it and no additional information, I don't think you can. Is there any way for you to also get information about the allele frequency of the variants you detected? I assume this was somatic tissue. Do you have access to data from blood samples or known normal tissue? Both additional data sources would help, but not be definitive. In the absence of additional data about your samples, you may want to try to annotate your variants, so that you can know if the variant is typically somatic or germ line, but this would require additional software, perhaps with access to the COSMIC database. From github, you can also download applications like, OncoGemini (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995589/). I hope this is helpful.
-
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
Comment