Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
Collapse
 
  • Page of 1
  • Filter
  • Time
  • Show
Clear All
new posts
Previous template Next
  • #1

    HISAT-3N: Finding Methylation Context

    I am a new user of HISAT-3N and am using it to analyze samples from a modified BS-seq experiment. I have generated the HISAT-3N conversion table which includes converted and unconverted counts by position. Is it possible to determine the context as I would like to differentiate CG, CHG or CHH? (I see one can specify CG only.) I note that other BS-seq packages like Bismarck provide this context.​ As I have built a pipeline using HISAT-3N, I would rather not start over with a different tool.

    Thanks in advance for you any help or advice.

    Best,

    Bryan
    Tags: None
Previous template Next

Latest Articles

Collapse
  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin




    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
    04-22-2024, 07:01 AM
  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM
View All

ad_right_rmr

Collapse

News

Collapse
Topics Statistics Last Post
Expanding the Horizons of Cellular Research with the Single Cell Atlas by seqadmin
Started by seqadmin, 04-25-2024, 11:49 AM
0 responses
15 views
0 likes
 Last Post seqadmin
by seqadmin
04-25-2024, 11:49 AM  
Genetic Variants and Diabetes Risk in Childhood Cancer Survivors by seqadmin
Started by seqadmin, 04-24-2024, 08:47 AM
0 responses
16 views
0 likes
 Last Post seqadmin
by seqadmin
04-24-2024, 08:47 AM  
Cancer Metastasis: A Deep Dive into Cellular Plasticity by seqadmin
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
62 views
0 likes
 Last Post seqadmin
by seqadmin
04-11-2024, 12:08 PM  
Proteogenomic Profiles Offer New Clues in Prostate Cancer by seqadmin
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
60 views
0 likes
 Last Post seqadmin
by seqadmin
04-10-2024, 10:19 PM  
View All
Working...
X