I am working with single-end RRBS data, but I do not have information about the restriction enzyme used during library preparation. Trim Galore offers the --rrbs option, which is tailored for data generated using the MspI enzyme. Is there a way to confirm from my raw FASTQ files whether MspI was used for restriction digestion?
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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