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Hello.
Are these results good for a library type fr-firststrand ?
I have a stranded library type and can't use library type UNSTRANDED.
so if the first strand is not good, then I need to use the second strand.
Thank you
Left reads:
Input : 30681699
Mapped : 13979559 (45.6% of input)
of these: 514390 ( 3.7%) have multiple alignments (4103 have >20)
Right reads:
Input : 30681699
Mapped : 13850738 (45.1% of input)
of these: 437904 ( 3.2%) have multiple alignments (4119 have >20)
45.4% overall read mapping rate.
Aligned pairs: 12729336
of these: 392039 ( 3.1%) have multiple alignments
77344 ( 0.6%) are discordant alignments
41.2% concordant pair alignment rate.
Are these results good for a library type fr-firststrand ?
I have a stranded library type and can't use library type UNSTRANDED.
so if the first strand is not good, then I need to use the second strand.
Thank you
Left reads:
Input : 30681699
Mapped : 13979559 (45.6% of input)
of these: 514390 ( 3.7%) have multiple alignments (4103 have >20)
Right reads:
Input : 30681699
Mapped : 13850738 (45.1% of input)
of these: 437904 ( 3.2%) have multiple alignments (4119 have >20)
45.4% overall read mapping rate.
Aligned pairs: 12729336
of these: 392039 ( 3.1%) have multiple alignments
77344 ( 0.6%) are discordant alignments
41.2% concordant pair alignment rate.
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