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  • lindenb
    replied
    Save your blast result as XML and process it with a XSLT stylesheet.

    Examples:

    * http://www.biostars.org/p/6635/

    * http://www.biostars.org/p/85258/

    Leave a comment:


  • gringer
    replied
    Use a different output format (ASN, CSV, or XML). The default text-based format is only meant to be for viewing, not data manipulation, and can change without notice.

    Leave a comment:


  • Genomics101
    started a topic Reformatting BLAST+ pairwise alignment?

    Reformatting BLAST+ pairwise alignment?

    Greetings!

    The default for BLAST+ output is a pairwise alignment broken up into 60bp increments:

    Code:
    Query  1     TTATTTTTCTTGATAGAATTAATTCTAATATTAGCACATTAAAAAATACTAAGTC-AAAT  59
                 ||||||| ||||||||  |||||| |||||||| |||||||||||||| |||||| ||||
    Sbjct  542   TTATTTTGCTTGATAGCGTTAATTATAATATTACCACATTAAAAAATATTAAGTCAAAAT  601
    
    Query  60    TGTAATATTTCATTTGATATTGTATGCATACAATTTT-ATC---T--TACCTTTAAGAAT  113
                 ||||||||||||||||||  ||||||||||||||||| |||   |  ||||||||| |||
    Sbjct  602   TGTAATATTTCATTTGAT-CTGTATGCATACAATTTTAATCTTATCATACCTTTAATAAT  660
    
    Query  114   ATGTATAA-TAATTTATACCCATGTAGTT-TATTAAAT--A-AACAT-ATTTGTGCGTAT  167
                 |||||||| |||| |||| | | ||| |   || ||||  |    || |||| | |||||
    Sbjct  661   ATGTATAATTAATATATATCAACGTATTCGAATCAAATGGATTTAATGATTTTTTCGTAT  720
    
    Query  168   CCAATGCGTTTATCTATTGTTATTACTA-T--TA-T--TATTGTTACTGCTATATCACTG  221
                   ||| |||||||||||||||||||||| |  || |  |||| ||| ||| || ||||||
    Sbjct  721   TTAATACGTTTATCTATTGTTATTACTATTACTATTACTATTATTATTGCCATCTCACTG  780
    I inquired with NCBI about a command for BLAST+ (neé StandALone BLAST) to have the aligment break at different increments or not at all. Like this:

    Code:
    Query  1     TTATTTTTCTTGATAGAATTAATTCTAATATTAGCACATTAAAAAATACTAAGTC-AAATTGTAATATTTCATTTGATATTGTATGCATACAATTTT-ATC---T--TACCTTTAAGAAT 113
                 ||||||| ||||||||  |||||| |||||||| |||||||||||||| |||||| ||||||||||||||||||||||  ||||||||||||||||| |||   |  ||||||||| |||
    Sbjct  542   TTATTTTGCTTGATAGCGTTAATTATAATATTACCACATTAAAAAATATTAAGTCAAAATTGTAATATTTCATTTGAT-CTGTATGCATACAATTTTAATCTTATCATACCTTTAATAAT  660
    They forwarded the request on to the development team, but don't have that functionality at the time in BLAST+. I was hoping someone might have had the same thought and wrote a little script to reformat these alignments?

    Thanks!
    Last edited by Genomics101; 12-04-2013, 04:42 PM.

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  • seqadmin
    Genetic Variation in Immunogenetics and Antibody Diversity
    by seqadmin



    The field of immunogenetics explores how genetic variations influence immune responses and susceptibility to disease. In a recent SEQanswers webinar, Oscar Rodriguez, Ph.D., Postdoctoral Researcher at the University of Louisville, and Ruben Martínez Barricarte, Ph.D., Assistant Professor of Medicine at Vanderbilt University, shared recent advancements in immunogenetics. This article discusses their research on genetic variation in antibody loci, antibody production processes,...
    11-06-2024, 07:24 PM

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