Save your blast result as XML and process it with a XSLT stylesheet.
Examples:
* http://www.biostars.org/p/6635/
* http://www.biostars.org/p/85258/
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Use a different output format (ASN, CSV, or XML). The default text-based format is only meant to be for viewing, not data manipulation, and can change without notice.
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Reformatting BLAST+ pairwise alignment?
Greetings!
The default for BLAST+ output is a pairwise alignment broken up into 60bp increments:
Code:Query 1 TTATTTTTCTTGATAGAATTAATTCTAATATTAGCACATTAAAAAATACTAAGTC-AAAT 59 ||||||| |||||||| |||||| |||||||| |||||||||||||| |||||| |||| Sbjct 542 TTATTTTGCTTGATAGCGTTAATTATAATATTACCACATTAAAAAATATTAAGTCAAAAT 601 Query 60 TGTAATATTTCATTTGATATTGTATGCATACAATTTT-ATC---T--TACCTTTAAGAAT 113 |||||||||||||||||| ||||||||||||||||| ||| | ||||||||| ||| Sbjct 602 TGTAATATTTCATTTGAT-CTGTATGCATACAATTTTAATCTTATCATACCTTTAATAAT 660 Query 114 ATGTATAA-TAATTTATACCCATGTAGTT-TATTAAAT--A-AACAT-ATTTGTGCGTAT 167 |||||||| |||| |||| | | ||| | || |||| | || |||| | ||||| Sbjct 661 ATGTATAATTAATATATATCAACGTATTCGAATCAAATGGATTTAATGATTTTTTCGTAT 720 Query 168 CCAATGCGTTTATCTATTGTTATTACTA-T--TA-T--TATTGTTACTGCTATATCACTG 221 ||| |||||||||||||||||||||| | || | |||| ||| ||| || |||||| Sbjct 721 TTAATACGTTTATCTATTGTTATTACTATTACTATTACTATTATTATTGCCATCTCACTG 780
Code:Query 1 TTATTTTTCTTGATAGAATTAATTCTAATATTAGCACATTAAAAAATACTAAGTC-AAATTGTAATATTTCATTTGATATTGTATGCATACAATTTT-ATC---T--TACCTTTAAGAAT 113 ||||||| |||||||| |||||| |||||||| |||||||||||||| |||||| |||||||||||||||||||||| ||||||||||||||||| ||| | ||||||||| ||| Sbjct 542 TTATTTTGCTTGATAGCGTTAATTATAATATTACCACATTAAAAAATATTAAGTCAAAATTGTAATATTTCATTTGAT-CTGTATGCATACAATTTTAATCTTATCATACCTTTAATAAT 660
Thanks!Last edited by Genomics101; 12-04-2013, 04:42 PM.
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The field of immunogenetics explores how genetic variations influence immune responses and susceptibility to disease. In a recent SEQanswers webinar, Oscar Rodriguez, Ph.D., Postdoctoral Researcher at the University of Louisville, and Ruben Martínez Barricarte, Ph.D., Assistant Professor of Medicine at Vanderbilt University, shared recent advancements in immunogenetics. This article discusses their research on genetic variation in antibody loci, antibody production processes,...-
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