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  • lucer105
    Member
    • Nov 2013
    • 12

    #1

    Problems when assembling the transcripts and count reads

    I load in bam files, and try to count reads for transcripts for edgeR and DEseq....

    ### call in the tools
    library(GenomicRanges)
    library(Rsamtools)
    library(DESeq)
    library(edgeR)
    library(org.Mm.eg.db)


    #####script begin
    txdb=makeTranscriptDbFromUCSC(genome='mm9',tablename='refGene')
    ex_by_gene=exonsBy(txdb,'gene')

    readsa=readBamGappedAlignments("a_accepted_hits.bam")

    countsa = countOverlaps(ex_by_gene,readsa)

    countTableabcd = data.frame(conditiona=countsa,conditionb=countsb,conditionc=countsc,conditiond=countsd,stringsAsFactors=FALSE)

    rownames(countTableabcd)=names(ex_by_gene)

    write.table(countTableabcd,file="countTableabcd.txt",sep="\t")

    ####### script end
    Error in as.vector(colSums(data)) :
    error in evaluating the argument 'x' in selecting a method for function 'as.vector': Error in colSums(data) : 'x' must be an array of at least two dimensions

    Could anyone explain what does the error reports mean? I guess that is the error form the step making "countsa", right? How to solve this?

    Thx,
  • lucer105
    Member
    • Nov 2013
    • 12

    #2
    Problem solved....................

    Comment

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