Hi, i use emboss getorf to extract orf, while i only want one longest sequences in my file, is there any available software of script can do this?
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I wrote a Python script (using Biopython) similar to getorf which has the option of returning all the longest ORFs (or the first longest ORF) in each sequence. This subtle distinction allows for the fact there may not be a single unique longest ORF, there could be several equally long ORFs.
It is available with a Galaxy wrapper here on the Galaxy Tool Shed:
Or, directly from GitHub:
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Thanks a lot , maubpComment
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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