Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Perl Help

    Hi All,

    When I upload the junction.bed file to UCSC, there was an error happen: "the chromosome name is case sensitive..." because name of chromosome is "1, 2, 3, 4,..." in my bowtie index.

    I wanted to change the name "1, 2, 3..." to "chr1, chr2, chr3...", then I use this code "perl -pi -e 's/^/chr/' filename" But I met an issue, since I run this code two times by accident , then the first string of each line of bed file became this embarrassing condition: "chrchr1,chrchr2,chrchr3..."

    Does anyone can help me to change it back to chr1.....? I am not familiar with Perl.

    Many thanks in advance!

  • #2
    perl -ane 's/chrchr/chr/g; print;' filename

    Comment


    • #3
      Thank you very much, RockChalkJayhawk, that works very well.

      Comment


      • #4
        Sorry, I am sort of silly at that time. I can just use text editor and then replace all.

        Comment


        • #5
          Originally posted by RockChalkJayhawk View Post
          perl -ane 's/chrchr/chr/g; print;' filename
          Or,

          perl -pe 's/chrchr/chr/g'

          accomplishes the same thing. -a isn't used in this context. and -p is like -n but prints every line.

          If your fingers are tired, might make a difference...

          --
          Phillip

          Comment


          • #6
            Originally posted by pmiguel View Post
            Or,

            perl -pe 's/chrchr/chr/g'

            accomplishes the same thing. -a isn't used in this context. and -p is like -n but prints every line.

            If your fingers are tired, might make a difference...

            --
            Phillip
            You can learn something new everyday. Thanks Phillip!

            Comment


            • #7
              Thanks RockChalkJayhawk and Phillip!

              Comment


              • #8
                Your original line probably should have been

                perl -pi -e 's/^([^c])/chr$1/'

                Comment


                • #9
                  Thanks Keith

                  Originally posted by krobison View Post
                  Your original line probably should have been

                  perl -pi -e 's/^([^c])/chr$1/'

                  Was just wondering.

                  Comment


                  • #10
                    Originally posted by krobison View Post
                    Your original line probably should have been

                    perl -pi -e 's/^([^c])/chr$1/'
                    or create a back up file in case the in place edit (-i) goes awry:

                    perl -i'.bak' -pe 's/^/chr/' file.bed

                    because if you find out you hosed file.bed, you can

                    mv file.bed.bak file.bed

                    to recover.

                    --
                    Phillip

                    Comment

                    Latest Articles

                    Collapse

                    • seqadmin
                      Advanced Tools Transforming the Field of Cytogenomics
                      by seqadmin


                      At the intersection of cytogenetics and genomics lies the exciting field of cytogenomics. It focuses on studying chromosomes at a molecular scale, involving techniques that analyze either the whole genome or particular DNA sequences to examine variations in structure and behavior at the chromosomal or subchromosomal level. By integrating cytogenetic techniques with genomic analysis, researchers can effectively investigate chromosomal abnormalities related to diseases, particularly...
                      Yesterday, 06:26 AM
                    • seqadmin
                      How RNA-Seq is Transforming Cancer Studies
                      by seqadmin



                      Cancer research has been transformed through numerous molecular techniques, with RNA sequencing (RNA-seq) playing a crucial role in understanding the complexity of the disease. Maša Ivin, Ph.D., Scientific Writer at Lexogen, and Yvonne Goepel Ph.D., Product Manager at Lexogen, remarked that “The high-throughput nature of RNA-seq allows for rapid profiling and deep exploration of the transcriptome.” They emphasized its indispensable role in cancer research, aiding in biomarker...
                      09-07-2023, 11:15 PM
                    • seqadmin
                      Methods for Investigating the Transcriptome
                      by seqadmin




                      Ribonucleic acid (RNA) represents a range of diverse molecules that play a crucial role in many cellular processes. From serving as a protein template to regulating genes, the complex processes involving RNA make it a focal point of study for many scientists. This article will spotlight various methods scientists have developed to investigate different RNA subtypes and the broader transcriptome.

                      Whole Transcriptome RNA-seq
                      Whole transcriptome sequencing...
                      08-31-2023, 11:07 AM

                    ad_right_rmr

                    Collapse

                    News

                    Collapse

                    Topics Statistics Last Post
                    Started by seqadmin, Today, 06:57 AM
                    0 responses
                    6 views
                    0 likes
                    Last Post seqadmin  
                    Started by seqadmin, Yesterday, 07:53 AM
                    0 responses
                    8 views
                    0 likes
                    Last Post seqadmin  
                    Started by seqadmin, 09-25-2023, 07:42 AM
                    0 responses
                    14 views
                    0 likes
                    Last Post seqadmin  
                    Started by seqadmin, 09-22-2023, 09:05 AM
                    0 responses
                    44 views
                    0 likes
                    Last Post seqadmin  
                    Working...
                    X