Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • Wei-HD
    Member
    • Oct 2009
    • 59

    #1

    Perl Help

    Hi All,

    When I upload the junction.bed file to UCSC, there was an error happen: "the chromosome name is case sensitive..." because name of chromosome is "1, 2, 3, 4,..." in my bowtie index.

    I wanted to change the name "1, 2, 3..." to "chr1, chr2, chr3...", then I use this code "perl -pi -e 's/^/chr/' filename" But I met an issue, since I run this code two times by accident , then the first string of each line of bed file became this embarrassing condition: "chrchr1,chrchr2,chrchr3..."

    Does anyone can help me to change it back to chr1.....? I am not familiar with Perl.

    Many thanks in advance!
  • RockChalkJayhawk
    Senior Member
    • Mar 2009
    • 192

    #2
    perl -ane 's/chrchr/chr/g; print;' filename

    Comment

    • Wei-HD
      Member
      • Oct 2009
      • 59

      #3
      Thank you very much, RockChalkJayhawk, that works very well.

      Comment

      • Wei-HD
        Member
        • Oct 2009
        • 59

        #4
        Sorry, I am sort of silly at that time. I can just use text editor and then replace all.

        Comment

        • pmiguel
          Senior Member
          • Aug 2008
          • 2328

          #5
          Originally posted by RockChalkJayhawk View Post
          perl -ane 's/chrchr/chr/g; print;' filename
          Or,

          perl -pe 's/chrchr/chr/g'

          accomplishes the same thing. -a isn't used in this context. and -p is like -n but prints every line.

          If your fingers are tired, might make a difference...

          --
          Phillip

          Comment

          • RockChalkJayhawk
            Senior Member
            • Mar 2009
            • 192

            #6
            Originally posted by pmiguel View Post
            Or,

            perl -pe 's/chrchr/chr/g'

            accomplishes the same thing. -a isn't used in this context. and -p is like -n but prints every line.

            If your fingers are tired, might make a difference...

            --
            Phillip
            You can learn something new everyday. Thanks Phillip!

            Comment

            • Wei-HD
              Member
              • Oct 2009
              • 59

              #7
              Thanks RockChalkJayhawk and Phillip!

              Comment

              • krobison
                Senior Member
                • Nov 2007
                • 734

                #8
                Your original line probably should have been

                perl -pi -e 's/^([^c])/chr$1/'

                Comment

                • Joann
                  Senior Member
                  • Oct 2008
                  • 230

                  #9
                  Thanks Keith

                  Originally posted by krobison View Post
                  Your original line probably should have been

                  perl -pi -e 's/^([^c])/chr$1/'

                  Was just wondering.

                  Comment

                  • pmiguel
                    Senior Member
                    • Aug 2008
                    • 2328

                    #10
                    Originally posted by krobison View Post
                    Your original line probably should have been

                    perl -pi -e 's/^([^c])/chr$1/'
                    or create a back up file in case the in place edit (-i) goes awry:

                    perl -i'.bak' -pe 's/^/chr/' file.bed

                    because if you find out you hosed file.bed, you can

                    mv file.bed.bak file.bed

                    to recover.

                    --
                    Phillip

                    Comment

                    Latest Articles

                    Collapse

                    • SEQadmin2
                      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
                      by SEQadmin2



                      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

                      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
                      ...
                      10-14-2026, 09:55 AM
                    • SEQadmin2
                      New Genomics Technologies Take Aim at Long-Standing Limits
                      by SEQadmin2


                      Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

                      We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
                      ...
                      09-28-2026, 10:25 AM

                    ad_right_rmr

                    Collapse

                    News

                    Collapse

                    Topics Statistics Last Post
                    Started by SEQadmin2, 10-15-2026, 10:52 AM
                    0 responses
                    15 views
                    0 reactions
                    Last Post SEQadmin2  
                    Started by SEQadmin2, 10-14-2026, 05:39 AM
                    0 responses
                    21 views
                    0 reactions
                    Last Post SEQadmin2  
                    Started by SEQadmin2, 10-13-2026, 09:56 AM
                    0 responses
                    21 views
                    0 reactions
                    Last Post SEQadmin2  
                    Started by SEQadmin2, 10-09-2026, 11:14 AM
                    0 responses
                    27 views
                    0 reactions
                    Last Post SEQadmin2  
                    Working...