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weird kmer in FastQC plot
I have performed whole exome sequencing using the 62Mb Nextera Exome Enrichment from Illumina. I have really weird results where a lot of the "aligned" reads have too many variants and so have checked the fastQC file and the kmer plots look really weird. Any ideas? Thanks.
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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
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Introduction
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