which software you use for extraction of the core genome "only house keeping genes" from bacterial genome (excleuding plasmids/ prophages/ ...).
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Some suggestions in this thread: http://seqanswers.com/forums/showthread.php?t=17530
For reference as you create a new thread look at the "similar threads window" that shows up above your edit window. Generally has suggestions for relevant threads that may answer your question.
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
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