Hello.
I am using trimmomatic and know that the sequences to be removed are
Adapter 5’ AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
>>TruSeq Adapter, Index 1
>>5’ GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTG
>>TruSeq Adapter, Index 2
>>5’ GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTG
>>TruSeq Adapter, Index 3
>>5’ GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTG
however I do not know how to create the adapter.fa file knowing just the sequence names.
Could anyone provide a sample adapter.fa file that they use as a adapter reference for trimming using the command ILLUMINACLIP ?
my other option is to use adaptercut, but I want to try the reference adapter.fa first...seems easier
I am using trimmomatic and know that the sequences to be removed are
Adapter 5’ AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
>>TruSeq Adapter, Index 1
>>5’ GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTG
>>TruSeq Adapter, Index 2
>>5’ GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTG
>>TruSeq Adapter, Index 3
>>5’ GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTG
however I do not know how to create the adapter.fa file knowing just the sequence names.
Could anyone provide a sample adapter.fa file that they use as a adapter reference for trimming using the command ILLUMINACLIP ?
my other option is to use adaptercut, but I want to try the reference adapter.fa first...seems easier
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