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RNA-seq depth for lncRNA quantification
HI, guys , i have 6 human cell samples preparing for RNA-seq to do mRNA expression and lncRNA expression meta-analysis. However, i am not sure how many reads or depth are enough to my analysis.
Someone told me that, for lncRNA analysis, there must be at least 9-10G raw data (PE100) by sequencing total RNA (only rRNA were removed). An alternative strategy is just sequencing the ployA enriched RNAs ,of which data volume should be at least 5G relatively (PE100).
Indeed, I just want perform an expression analysis without novel lncRNA detection, so I think the SE50 can be suitable for quatifing those known lncRNA transcripts. Could anyone can make me know the minimum reads number or depth to do this work by SE50 in either strategy mentioned above.
Many thanks!
Someone told me that, for lncRNA analysis, there must be at least 9-10G raw data (PE100) by sequencing total RNA (only rRNA were removed). An alternative strategy is just sequencing the ployA enriched RNAs ,of which data volume should be at least 5G relatively (PE100).
Indeed, I just want perform an expression analysis without novel lncRNA detection, so I think the SE50 can be suitable for quatifing those known lncRNA transcripts. Could anyone can make me know the minimum reads number or depth to do this work by SE50 in either strategy mentioned above.
Many thanks!
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