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  • question about coverage

    Hi:

    We are planning a new experiment to identify a mutations using big blue mouse with lacz insertion. the expected frequency of mutations are 10-6. the proposed sequencing of the Insert size is 1kb region. Using HiSeq 2500 two lanes, given each lane can generate ~200Million reads, and at 33 Phred score quality of base in 100 read length (we will chop first and last 25 reads in a 150 read length read) gives an error 1 in more than 10,000.

    Is this doable. Where can I find a method to do this.

    Thanks

  • #2
    Any suggestions please...thanks

    Comment


    • #3
      I'd do paired end sequencing of short fragments, so that the two reads overlap completely and generate Phred scores of 60-70.
      Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.com

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