Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • annotation with prokka

    Hello world,

    This would be a silly question but could someone help me understand what prokka is doing? As far as I know, I can use assembled config fasta file to blast. For example, if I have bacterial nucleotide sequence, I can use blatx program then my nucleotide sequence is converted to protein sequences and they are searched through protein database in genbank. It is said that prokka output could be used for genbank submission. Why do we want to use prokka? Any comment would be greatly appreciated.

  • #2
    Prokka is a pipeline to predict genomic elements, such as genes, rRNA, tRNA and non-coding RNA. In addition, it annotates the genes with BLAST against the swissprot database and other databases. Prokka generates files that can directly be used for submission in GenBank.

    Regards,
    Boetsie

    Comment


    • #3
      Dear boetsie,

      Thanks a lot!

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Current Approaches to Protein Sequencing
        by seqadmin


        Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
        04-04-2024, 04:25 PM
      • seqadmin
        Strategies for Sequencing Challenging Samples
        by seqadmin


        Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
        03-22-2024, 06:39 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 04-11-2024, 12:08 PM
      0 responses
      18 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 10:19 PM
      0 responses
      22 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 09:21 AM
      0 responses
      17 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-04-2024, 09:00 AM
      0 responses
      48 views
      0 likes
      Last Post seqadmin  
      Working...
      X