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  • bio_informatics
    Senior Member
    • Nov 2013
    • 182

    issues in running prof protein prediction tool

    I have profphd installed successfully in usr/path.

    When I run
    prof /usr/share/profphd/prof/exa/1ppt.f
    I get correct file.
    But when I try to run,

    prof sra.fas 3 doHtmtop
    where sra.fas is the input fasta file, I get the below error.
    substr outside of string at /usr/share/perl5/RG/Utils/Copf.pm line 2401, <FHIN_convFastamul2msf> line 70.
    Use of uninitialized value in concatenation (.) or string at /usr/share/perl5/RG/Utils/Copf.pm line 2401, <FHIN_convFastamul2msf> line 70.
    substr outside of string at /usr/share/perl5/RG/Utils/Copf.pm line 2401, <FHIN_convFastamul2msf> line 70.
    Use of uninitialized value in concatenation (.) or string at /usr/share/perl5/RG/Utils/Copf.pm line 2401, <FHIN_convFastamul2msf> line 70.
    *** ERROR profrotRd: after protRdAli, fileIn=sra.fas,
    *** message from sbr:
    *** ERROR protRdAli: failed conversion to HSSP
    ok lib-col:sysRunProg

    *** ERROR prof: line number of error=113
    *** ERROR failed prof:full:doOne dbfile=sra.fas, chain=*!
    *** ERROR msg from where it failed:
    *** ERROR doOne: reading db=sra.fas, chain=*! (&protRd)
    line=1436
    *** ERROR protRd: *** ERROR profrotRd: after protRdAli, fileIn=sra.fas,
    *** message from sbr:
    *** ERROR protRdAli: failed conversion to HSSP
    ok lib-col:sysRunProg

    *** ERROR doOne: reading db=sra.fas, chain=*! (&protRd)
    line=1436
    *** ERROR protRd: *** ERROR profrotRd: after protRdAli, fileIn=sra.fas,
    *** message from sbr:
    *** ERROR protRdAli: failed conversion to HSSP
    ok lib-col:sysRunProg
    at /usr/share/profphd/prof/scr/lib/lib-prof.pl line 107.
    prof::assAbort("failed prof:full:doOne dbfile=sra.fas, chain=*!", 113, "*** ERROR doOne: reading db=sra.fas, chain=*! (&protRd)\x{a}line="...) called at /usr/share/profphd/prof/scr/lib/prof.pm line 112
    prof::full("/usr/share/profphd/", "/usr/share/profphd/prof/", "/usr/share/profphd/prof/scr/CONFprof.pl", "LINUX", "prof", "neural network switching", "list_of_files (or single file) parameter_file", 2, "hssp,dssp,msf,saf,fastamul,pirmul,fasta,pir,gcg,swiss", ...) called at /usr/bin/prof line 170
    I cannot understand how I to give an input file which is not present in
    /usr/share/profphd/prof
    and get an output.

    Please help me.
    Bioinformaticscally calm

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