Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • barkasn
    Junior Member
    • Mar 2012
    • 9

    Cuffdiff 2 progress reporting

    Hi All,

    I am running cuffdiff2 on 2 samples with 4 replicates each and a total of 160m reads (PE 80bp) for nearly 48 hours now, however I don't get any feedback about progress and I have no way of determining if this is normal operation or if cuffdiff is stuck.

    The program is using 100% of 1 CPU and 0% memory (despite specifying -p 7) and the only output so far has been "You are using Cufflinks v2.1.1, which is the most recent release." on stderr. A number of files have been created in the output directory but they are all empty.

    Is this normal for this version of cuffdiff? What are your experiences?

    Thanks in advance,

    barkasn
    Last edited by barkasn; 01-09-2014, 02:47 AM.
  • TiborNagy
    Senior Member
    • Mar 2010
    • 329

    #2
    It is absolutly normal if you do not have free disk space :-)

    Comment

    • barkasn
      Junior Member
      • Mar 2012
      • 9

      #3
      The issue had to do with the script calling cuffdiff, it would provide the path to the samfiles but not the actual name. Cuffdiff did not display an error message and kept on running using a single processor. This is a bug really and should be fixed.

      Comment

      Latest Articles

      Collapse

      • GATTACAT
        Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
        by GATTACAT
        Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
        07-01-2026, 11:43 AM
      • SEQadmin2
        Nine Things a Sample Prep Scientist Thinks About Before Sequencing
        by SEQadmin2


        I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

        Here are nine questions we think about, in roughly the order they matter, before...
        06-18-2026, 07:11 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, 07-02-2026, 11:08 AM
      0 responses
      26 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-30-2026, 05:37 AM
      0 responses
      24 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-26-2026, 11:10 AM
      0 responses
      23 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-17-2026, 06:09 AM
      0 responses
      55 views
      0 reactions
      Last Post SEQadmin2  
      Working...