De novo MHC/multi-template genotyping using MiSeq

[JackieBadger]

There has been some discussion on here regarding MHC genotyping using next-gen, so I thought I would share our recent contribution:

http://onlinelibrary.wiley.com/doi/1...12225/abstract

The rational can be applied to genotyping any multi-template amplicon e.g. sequenced micro-sats, and helps to deal with cross-sample contamination (or stutter in micros) that is common in such approaches.

Cheers,

J

[JackieBadger]

As a follow up, if you are interested please see our recent review about using NGS for de novo multigene family genotyping:

http://onlinelibrary.wiley.com/doi/1...12843/abstract

Thanks