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  • libro.s
    Junior Member
    • Jun 2010
    • 2

    #1

    non balanced design in DESeq2

    Hi,
    I apologize in advance if this question has been already asked but I`m wondering if DESeq2 can handle a non factorial design. Let`s say I have 2 factors A and B, A has 2 levels and B has 3. Each of the 2 levels of A is treated with each level of B, except 2 samples where only one level of B could be recovered. One option is obviously to exclude those 2 samples, but I was wondering if there is a way to include them in the analysis and increase the sample size.
    Thank you in advance!

    Silvia
  • dpryan
    Devon Ryan
    • Jul 2011
    • 3478

    #2
    You can do incomplete factorial designs with DESeq (or edgeR or limma).

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