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  • id0
    Senior Member
    • Sep 2012
    • 130

    #1

    ReportingTools publish with multiple factors

    I just started using DESeq2. One of the really nice features I discovered is I can use the DESeqDataSet object with ReportingTools to generate charts of all of the significant genes. However, I can't figure out how to properly handle multi-factor designs.

    With a single factor, it's simple:
    Code:
    publish(dds, Report, factor = colData(dds)$A, ...)
    This works with multi-factor designs, but outputs charts only with 2 conditions from A.

    I tried combining the factors:
    Code:
    f <- factor(c(as.character(colData(dds)$A),as.character(colData(dds)$B)))
    publish(dds, Report, factor = f, ...)
    I got output with all conditions (2 from A and 2 from B), but all the values were for the 2 from A. It makes sense based on my code (it's just a factor with 4 unique values), but not the answer I was looking for.

    I also tried generating a factor matrix, but that yielded an error for charting:
    Code:
    m <- cbind(as.character(colData(dds)$Diet),as.character(colData(dds)$Age))
    dm <- as.data.frame(m)
    dm[,1] <- as.factor(dm[,1])
    dm[,2] <- as.factor(dm[,2])
    publish(dds, Report, factor = dm, ...)
    Is it possible to combine factors with ReportingTools?
    Last edited by id0; 01-10-2014, 10:24 AM.

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