Hi All,
I used the fastQC kit to determine read quality of my libraries and everything looked good except for the level of duplication, which was very high in all of my libraries (see attached pic).
It seems to me that having such a high level of duplication would affect differential gene expression analysis. My question is, is this something I should be worried about? And if so what can I do to fix the issue.
thanks
Mila
I used the fastQC kit to determine read quality of my libraries and everything looked good except for the level of duplication, which was very high in all of my libraries (see attached pic).
It seems to me that having such a high level of duplication would affect differential gene expression analysis. My question is, is this something I should be worried about? And if so what can I do to fix the issue.
thanks
Mila
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