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Hi All,
I am analyzing sRNA data of mouse from Illumina. I have successfully completed the quality checks, pre-processing and identified differentially expressed sRNAs from my data.
I would like to annotate the differentially expressed snRNA and snoRNA from the list of differentially expressed sRNA. For doing so I downloaded annotations from useast.enseml.org (http://useast.ensembl.org/info/data/ftp/index.html) but the length of the snRNA and snoRNA are 60bp to 150 bp, which is longer than maximum read length i.e 34bp in sRNA data anlysed. Can anybody tell me is there any way I could find and download mature snRNA and snoRNA sequences which are not longer than 34bp and could be used for annotation?
Please suggest
DAnand
I am analyzing sRNA data of mouse from Illumina. I have successfully completed the quality checks, pre-processing and identified differentially expressed sRNAs from my data.
I would like to annotate the differentially expressed snRNA and snoRNA from the list of differentially expressed sRNA. For doing so I downloaded annotations from useast.enseml.org (http://useast.ensembl.org/info/data/ftp/index.html) but the length of the snRNA and snoRNA are 60bp to 150 bp, which is longer than maximum read length i.e 34bp in sRNA data anlysed. Can anybody tell me is there any way I could find and download mature snRNA and snoRNA sequences which are not longer than 34bp and could be used for annotation?
Please suggest
DAnand