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Hi there!!
I want to detect differential expression of no-coding Rna from two type of cells. I have a stranded RNA-seq library. I have alligned using tophat on trascriptome and on ncRNa.fasta.
I trye to create count of ncRNA but I wan not able to abtain that using htseq-count ( allthings go on not annotated..) Have you a suggestion on how to resolve? How can create a bed file starting from databese of ncRNA of ensemble? Thanks a lot!
I want to detect differential expression of no-coding Rna from two type of cells. I have a stranded RNA-seq library. I have alligned using tophat on trascriptome and on ncRNa.fasta.
I trye to create count of ncRNA but I wan not able to abtain that using htseq-count ( allthings go on not annotated..) Have you a suggestion on how to resolve? How can create a bed file starting from databese of ncRNA of ensemble? Thanks a lot!