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Hello to all NGS experts,
Just a quick question from your experience.
I would like to align my small RNA reads of SOLiD color space data with bowtie. Therefore, its worth to define --col-keepends (to keep first and last color after alignment) flag that bowtie suppose not to have as default. ???
In my point of view bowtie deal with, in right way but in tophat having --col-keepends flag as default and suppose to have first (T) and last color. Why exactly i don't understand ??
May i should have this option --col-keepends or not ??? what is the benefit or disadvantage if i don't have this option ??
Help me plsss ??? to short-out my doubt ???
Just a quick question from your experience.
I would like to align my small RNA reads of SOLiD color space data with bowtie. Therefore, its worth to define --col-keepends (to keep first and last color after alignment) flag that bowtie suppose not to have as default. ???
In my point of view bowtie deal with, in right way but in tophat having --col-keepends flag as default and suppose to have first (T) and last color. Why exactly i don't understand ??
May i should have this option --col-keepends or not ??? what is the benefit or disadvantage if i don't have this option ??
Help me plsss ??? to short-out my doubt ???