Pileup Format Question: Quality Scores

[IMPERATOR]

Hi all,

So I'm a little lost on the quality scores in the pileup files I have generated.

From what I read, it is the ASCII value minus 33. So I'm assuming it's the decimal ASCII value?

Or is there an easier way to convert the glyphs into phred integers?

TUSEN TAKK

[dpryan]

The normal way to phrase it is that the ASCII values are 33 plus the phred score. So, a phred score of 30 would lead to 30+33=63='?'

[IMPERATOR]

So it's decimal. THANKS!

[GenoMax]

Here is the encoding info: http://en.wikipedia.org/wiki/FASTQ_format#Encoding