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  • mmmm
    Senior Member
    • Jul 2013
    • 131

    #1

    fastq-mcf

    using fastq-mcf for adapters trimming and removing low quality read (<20)- but have got this during the analysis??
    Within-read Skew: Position 14 from the start of reads is skewed!
    Within-read Skew: Position 46 from the start of reads is skewed!
    Within-read Skew: Position 47 from the start of reads is skewed!
    Within-read Skew: Position 48 from the start of reads is skewed!
    Within-read Skew: Position 49 from the start of reads is skewed!
    Within-read Skew: Position 6 from the end of reads is skewed!
    Within-read Skew: Position 8 from the end of reads is skewed!
    Within-read Skew: Position 12 from the end of reads is skewed!
    Within-read Skew: Position 14 from the end of reads is skewed!
    Within-read Skew: Position 15 from the end of reads is skewed!
    Within-read Skew: Position 17 from the end of reads is skewed!
    Within-read Skew: Position 18 from the end of reads is skewed!
    Within-read Skew: Position 22 from the end of reads is skewed!
    Within-read Skew: Position 27 from the end of reads is skewed!
    Within-read Skew: Position 28 from the end of reads is skewed!
    Within-read Skew: Position 29 from the end of reads is skewed!
    Within-read Skew: Position 30 from the end of reads is skewed!
    Within-read Skew: Position 31 from the end of reads is skewed!
    Within-read Skew: Position 32 from the end of reads is skewed!
    Within-read Skew: Position 33 from the end of reads is skewed!
    Within-read Skew: Position 34 from the end of reads is skewed!
    Within-read Skew: Position 35 from the end of reads is skewed!
    Within-read Skew: Position 36 from the end of reads is skewed!
    Within-read Skew: Position 47 from the end of reads is skewed!
    Within-read Skew: Position 5 from the start of reads is skewed!
    Within-read Skew: Position 6 from the start of reads is skewed!
    Within-read Skew: Position 8 from the start of reads is skewed!
    Within-read Skew: Position 9 from the start of reads is skewed!
    Within-read Skew: Position 10 from the start of reads is skewed!
    Within-read Skew: Position 11 from the start of reads is skewed!
    Within-read Skew: Position 12 from the start of reads is skewed!
    Within-read Skew: Position 10 from the end of reads is skewed!
    Within-read Skew: Position 11 from the end of reads is skewed!
    Within-read Skew: Position 32 from the end of reads is skewed!
    Within-read Skew: Position 36 from the end of reads is skewed!

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