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  • charlesxu90
    Junior Member
    • Jan 2013
    • 3

    #1

    How to select representative sequence from multiple sequence alignment

    Hi all,

    I'm working on a project which needs to find a representative sequence from multiple sequence alignment result (from clustalw).

    My problem is like this:

    This are aligned sequences:
    ATCGACGTAC
    A-CGA-GTAG
    A-CGA-CTAC
    A-CGA-GTAC

    This is what I want:
    ACGAGTAC

    Which returns the mode of each base. If the mode of a base is a gap, then return the gap.
  • TiborNagy
    Senior Member
    • Mar 2010
    • 329

    #2
    You can use showalign from the Emboss package to get consensus sequence.

    Comment

    • charlesxu90
      Junior Member
      • Jan 2013
      • 3

      #3
      Originally posted by TiborNagy View Post
      You can use showalign from the Emboss package to get consensus sequence.
      I tried but couldn't install Emboss package. It might be out of date, and cannot be downloaded now.

      Comment

      • GenoMax
        Senior Member
        • Feb 2008
        • 7142

        #4
        Originally posted by charlesxu90 View Post
        I tried but couldn't install Emboss package. It might be out of date, and cannot be downloaded now.
        Did you try to download from this site: ftp://emboss.open-bio.org/pub/EMBOSS/
        Latest version is 6.6.0 released July 2013.

        Comment

        • charlesxu90
          Junior Member
          • Jan 2013
          • 3

          #5
          Originally posted by GenoMax View Post
          Did you try to download from this site: ftp://emboss.open-bio.org/pub/EMBOSS/
          Latest version is 6.6.0 released July 2013.
          It works. Thank you.

          Comment

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