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  • Need Help Regarding Reciprocal best hit in BLAST

    Hello everyone

    Greetings

    I need to know about the syntax used for BLAST using Reciprocal Best Hit method. What parameters should I use for it.
    If you have some time.... then please provide me links for reading about Reciprocal Best Hit method.

    Thanks in anticipation
    Regards from
    Bharat

  • #2
    I don't understand your question. Reciprocal Best Hit isn't something built into the BLAST tools. Most people doing a BLAST Reciprocal Best Hit (RBH) do this with a script in Perl/Python/... You just run BLAST with suitable parameters twice, first using list A as the queries and list B as the database, and then the other way round.

    You should be able to find examples of BLAST RBH in the literature.

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