Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • AnushaC
    Member
    • Sep 2013
    • 78

    #1

    Interconnecting 2 to 3 shell scripts

    Hi All,
    I have 3 to 4 working shell scripts
    for SRA to FASTQ
    FASTQ to BAM
    BAM to BEDGRAPH
    can I write single shell script interconnecting these shell which are working individually fine . If so it is creating me several problem like I am writing fastq files to a temporary folder which is not able is recognize by second shell . If there any online links to these kind of problems I would appreciate help. What are kinds of issues to consider while merging several shell programs .

    Thanks in advance,
    Anusha.Ch
  • TiborNagy
    Senior Member
    • Mar 2010
    • 329

    #2
    Yes, you can write a single shell script wich is contains only other shell scripts. You need to set the command line parameters to each script get a proper input files. For example:
    #!/bin/bash

    ./first.sh input.sra >/tmp/out.fastq
    ./second.sh /tmp/out.fastq >/tmp/out.bam

    Even better, if you use variables, because there is less chance to misstype. For example:
    FASTQ=/tmp/out.fastq
    ./fist.sh input.sh >$FASTQ
    ./second.sh $FASTQ ...

    Comment

    • gringer
      David Eccles (gringer)
      • May 2011
      • 845

      #3
      If you want to conserve space and get something close to pipe-like functionality, you can use FIFO buffers:

      Code:
      $ mkfifo test.txt
      $ cat test.txt &
      [1] 21854
      $ echo -e "blah\nblah\nblah" > test.txt
      $ blah
      blah
      blah
      
      [1]+  Fertig                  cat test.txt

      Comment

      Latest Articles

      Collapse

      • SEQadmin2
        Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
        by SEQadmin2



        Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

        Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
        ...
        10-14-2026, 09:55 AM
      • SEQadmin2
        New Genomics Technologies Take Aim at Long-Standing Limits
        by SEQadmin2


        Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

        We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
        ...
        09-28-2026, 10:25 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, Yesterday, 11:13 AM
      0 responses
      19 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, Yesterday, 10:48 AM
      0 responses
      18 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-15-2026, 10:52 AM
      0 responses
      36 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-14-2026, 05:39 AM
      0 responses
      42 views
      0 reactions
      Last Post SEQadmin2  
      Working...