Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • mamonster
    Junior Member
    • Feb 2014
    • 2

    #1

    Filling Gaps between Chimeric junction sites

    Hi,

    I was wondering if there is well established method to find the entire transcripts (with exact alternative spliced sequences) formed from the chimeric junctions?
    Alignment tools such as STAR and Tophat do provide methods to detect chimeric junction sites from RNA-Seq data. However I couldn't find any obvious solution to assemble the exact isoforms. To be specific, suppose I had a bed file, contains all the potential chimeric junction sites found by the aligners from a RNA-Seq data set(with reference genome), how do I find the exact sequence between the junctions?

    Thanks

Latest Articles

Collapse

  • kinpict.com
    Reply to 16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
    by kinpict.com
    The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.
    Today, 06:37 PM
  • SEQadmin2
    16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
    by SEQadmin2





    NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
    10-30-2026, 06:46 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 11-12-2026, 07:59 AM
0 responses
27 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 11-05-2026, 09:25 AM
0 responses
35 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 11-03-2026, 11:54 AM
2 responses
56 views
0 reactions
Last Post kinpict.com  
Started by SEQadmin2, 10-28-2026, 09:57 AM
0 responses
175 views
0 reactions
Last Post SEQadmin2  
Working...