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  • anibhax
    Member
    • Jan 2013
    • 24

    #1

    Need help explaining the output of link files generated using SVDetect

    Hi,

    I have used SVDetect to generate links. And I got it converted to circos input format (segdup.txt). I don't understand the logic of this format. Here are the first few lines:

    Code:
    1    hschr1    10000005    10000080    color=white
    1    hschr18    54254700    54254735    color=white
    2    hschr1    10000008    10000083    color=white
    2    hschr18    19966691    19966726    color=white
    3    hschr1    10000016    10000051    color=white
    3    hschr16    66915746    66915821    color=white
    4    hschr1    10000016    10000051    color=grey
    4    hschr17    29951284    29951359    color=grey
    5    hschr1    10000016    10000051    color=white
    5    hschrX    15854763    15854838    color=white
    Why is the color important? I understand it has something to do with generating circos plots. Nothing more. And I also noticed that only about 8 colors are present.

    Any help making me understand this output is appreciated. I have looked everywhere, but I never any page explaining these colors.

    Thanks!
  • TiborNagy
    Senior Member
    • Mar 2010
    • 329

    #2
    The first column is the link ID. So two line identify one connection between two positions. The reason behind this is the flexibility. You can create a link between three or more position. The color is simply the color of the connection line in the final image. Circos has a very few default values, so you need to define every parameter.

    Comment

    • anibhax
      Member
      • Jan 2013
      • 24

      #3
      Can you elaborate on that? The first two rows have the same id's: 1. So I'm assuming this means that links exist between chr1 and chr18. Does this mean it is a inter-chromosomal link? And do you know from which position to which position the link is drawn in this case?

      Thanks!

      Comment

      • TiborNagy
        Senior Member
        • Mar 2010
        • 329

        #4
        Yes, the first two rows are an interchromosomal link. The positions can be found in the 3th and 4th columns. Actually it is not a single position, but a region.

        Comment

        • anibhax
          Member
          • Jan 2013
          • 24

          #5
          That makes sense. Thanks!

          Comment

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