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  • inscent
    Junior Member
    • Jun 2013
    • 2

    #1

    Running TopHat/Cufflinks on Google Compute Engine

    Does anyone have any experience using the Google Compute Engine (or Amazon Web Services, or any other IAAS for that matter) to do RNA-Seq analysis with TopHat? The computers I have available are not at all suited to the task, and so I'd be interested to hear from anyone who has used one of these services.
  • scbody
    Junior Member
    • Dec 2013
    • 2

    #2
    GoogleCompute

    Hi
    I'm also interested in hearing of anyone doing analyses on GoogleCompute. It seems much more functional and certainly cheaper now.
    Thanks

    Comment

    • mastal
      Senior Member
      • Mar 2009
      • 666

      #3
      I used Amazon Web Services EC2 to analyze some RNA-Seq data with Bowtie, Tophat and Cufflinks a few years ago.

      I used a Ubuntu Linux ami, with 16 Gigabytes of ram, which doesn't seem like a lot now. I had files with 90 million 1X50 reads. I had started off with either a
      4 Gb or 8 Gb machine, but got segmentation faults.

      I just used a basic Linux ami, so I had to install all the bioinformatics software i wanted to use, like the Bowtie, Tophat, etc.

      Comment

      • scbody
        Junior Member
        • Dec 2013
        • 2

        #4
        Google Compute

        Thanks for the information inscent.
        Does anyone have information on recent use of the Google Compute?
        Thank you

        Comment

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