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  • bongbimit
    Member
    • Dec 2013
    • 29

    #1

    error alignRead.pl in Trinity

    dear all,
    In step aligning read back to Trinity.fasta(transcriptome assembled) to count how many read 's assembed, i use alignRead.pl in Trinity
    but i got a error
    alignReads.pl --seqType fq --left data/TrinityNatureProtocolTutorial/1M_READS_sample/reads.all.left.fq --rigth data/TrinityNatureProtocolTutorial/1M_READS_sample/reads.all.right.fq --SS_lib_type RF --retain_intermediate_files --aligner bowtie --target data/TrinityNatureProtocolTutorial/assembly/ref_tran.fasta/Trinity.fasta -- -p 4 &
    -> error
    sorry, cannot find data/TrinityNatureProtocolTutorial/1M_READS_sample/reads.all. left.fq and at /home/huypham/tools/denovotranscriptome/trinityrnaseq_r20131110/ util/alignReads.pl line 198.
    how can i fix this?
    tks in advance
  • TiborNagy
    Senior Member
    • Mar 2010
    • 329

    #2
    Try to use absolute path in the command line.

    Comment

    • bongbimit
      Member
      • Dec 2013
      • 29

      #3
      still error

      Comment

      • TiborNagy
        Senior Member
        • Mar 2010
        • 329

        #4
        I still beleve you use wrong path. I have checked the source of alignReads.pl and that line just check the existence of files. Maybe there is a wrong symlink, a slow mounted drive, network filesistem, wrong file permissions, etc.

        Comment

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