Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • madkitty
    Junior Member
    • Feb 2014
    • 1

    #1

    cummerBund -> Doesn't see my samples

    I have 3 samples in 3 folders under the working directory of R, I did "Change directory" and pointed out one new folder "data" under the root C. "Data" contains our 3 samples outputed from cuffdiff.
    • ~/Sample1
    • ~/Sample2
    • ~/Sample3


    How can I point to R the directory for each sample in one readCufflinks command?
    If I just call readCufflinks() it returns 0 sample ..

    Code:
         > cuff<-readCufflinks()
         > cuff
         CuffSet instance with:
             0 samples
             0 genes
             0 isoforms
             0 TSS
             0 CDS
             0 promoters
             0 splicing
             0 relCDS


    I need to execute the following, and retrieve cuff, dispersion graph, density graph and boxplot graph:

    Code:
    > cuff<-readCufflinks(system.file("extdata", package="cummeRbund"))
    > cuff 
    
    > disp<-dispersionPlot(genes(cuff))
    > disp
    
    > genes.scv<-fpkmSCVPlot(genes(cuff)) 
    > isoforms.scv<-fpkmSCVPlot(isoforms(cuff))
    
    > dens<-csDensity(genes(cuff)) 
    > dens
    
    > b<-csBoxplot(genes(cuff)) 
    > b
  • TiborNagy
    Senior Member
    • Mar 2010
    • 329

    #2
    Code:
    setwd('Sample1')
    cuff1 <- readCufflinks()
    setwd('../Sample2')
    cuff2 <- readCufflinks()
    setwd('../Sample3')
    cuff3 <- readCufflinks()

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM
    • SEQadmin2
      New Genomics Technologies Take Aim at Long-Standing Limits
      by SEQadmin2


      Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

      We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
      ...
      09-28-2026, 10:25 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 10-22-2026, 11:13 AM
    0 responses
    26 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-22-2026, 10:48 AM
    0 responses
    20 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-15-2026, 10:52 AM
    0 responses
    36 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-14-2026, 05:39 AM
    0 responses
    43 views
    0 reactions
    Last Post SEQadmin2  
    Working...