Chip-SEq Data Analysis. S. cerevisiae.IOn-Torrent

[mpalegre]

Hi everybody. I´m Mónica from CABIMER (SEville, Spain). I´m trying to analyze Chip-SEq Data of S. cerevisiae provided by Ion-Torrent PGM. I have used MACS in Galaxy but I get a few peaks (I have Chip & control). I´m not sure if the parameters I am using are correct. i.e. Tag (=Reads): 156, BW= 300. If i don´t use "model" I have some peaks more, but which is the "shift size" better?. Can Anyone help me? Must I use other software/algorithm?

Thanks in advance

Regards

[crazyhottommy]

Hi Monica, you should read the MACS paper carefully and understand the parameters.
the MACS google group is also very helpful https://groups.google.com/forum/#!fo...s-announcement