Unconfigured Ad

Collapse
X
Collapse
 
  • Page of 1
  • Time
  • Show
Clear All
new posts
Previous template Next
  • Parharn
    Member
    • Jul 2013
    • 84
    #1

    How many replicates, how many reads for S. pombe RNA-seq

    Hi,
    I am planning to do RNA-seq on S. pombe. I would like to have a good overview of how many reads and replicates do I need to get confidential sequencing.
    Please leave your suggestions or link to any well explained publications for the issue.

    Thanks,
    Parham
    Tags: None
    • TiborNagy
      Senior Member
      • Mar 2010
      • 329
      #2
      This is a good explanation of you problem:
      pubmed.ncbi.nlm.nih.gov
      http://www.ncbi.nlm.nih.gov/pubmed/24319002

        Comment

        Previous template Next

        Latest Articles

        Collapse
        • SEQadmin2
          16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
          by SEQadmin2





          NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
          10-30-2026, 06:46 AM
        • SEQadmin2
          Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
          by SEQadmin2



          Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

          Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating          ...
          10-14-2026, 09:55 AM
        View All

        ad_right_rmr

        Collapse

        News

        Collapse
        Topics Statistics Last Post
        Gene Copies Once Dismissed as Junk DNA Shape Animal Adaptation and Human Disease by SEQadmin2
        Started by SEQadmin2, Today, 07:59 AM
        0 responses
        13 views
        0 reactions
        		 Last Post SEQadmin2
        by SEQadmin2
        Today, 07:59 AM  
        WashU Medicine Study Verifies Methods for Tracking Genetic Mosaicism by SEQadmin2
        Started by SEQadmin2, 11-05-2026, 09:25 AM
        0 responses
        32 views
        0 reactions
        		 Last Post SEQadmin2
        by SEQadmin2
        11-05-2026, 09:25 AM  
        HuBMAP Portal Offers Free Access to Single-Cell Data from Healthy Human Tissues by SEQadmin2
        Started by SEQadmin2, 11-03-2026, 11:54 AM
        0 responses
        45 views
        0 reactions
        		 Last Post SEQadmin2
        by SEQadmin2
        11-03-2026, 11:54 AM  
        Genome Sequencing Reveals Large, Repeat-Rich DNA in Trapdoor Spider Named for Stephen Colbert by SEQadmin2
        Started by SEQadmin2, 10-28-2026, 09:57 AM
        0 responses
        170 views
        0 reactions
        		 Last Post SEQadmin2
        by SEQadmin2
        10-28-2026, 09:57 AM  
        View All
        Working...