I am trying to assemble reads from an ssRNA virus into a reference genome. In this case, does strand bias apply ? Also, how should the second column of the Bowtie output "Reference strand(+/-) of the reference genome" be interpreted ? Should I discard all the - rows and only keep rows that align to the + strand of the reference ? Maybe I don't understand RNA and cDNA biology enough. Could someone please explain ?
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM -
Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.
The systematic characterization of the human proteome has...07-20-2026, 11:48 AM
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