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  • capricy
    Senior Member
    • Apr 2012
    • 125

    #1

    tophat error: Converting left_kept_reads.m2g to genomic coordinates (map2gtf), Failed

    I ran tophat with the following commandline:
    tophat2.0.0 -G genome.gff3 -o -o thout -r 180 genome2 seq1 seq2

    and exited with the following error:
    ----
    [2014-03-07 23:35:21] Converting left_kept_reads.m2g to genomic coordinates (map2gtf)
    [FAILED]
    Error: map2gtf returned an error
    ----

    could someone please help me out? Thanks a lot for your time!
  • capricy
    Senior Member
    • Apr 2012
    • 125

    #2
    Typo in the commandline. It should be:

    tophat2.0.0 -G genome.gff3 -o thout -r 180 genome2 seq1 seq2

    Comment

    • capricy
      Senior Member
      • Apr 2012
      • 125

      #3
      also, if -G genome.gff3 was not there, like only running the following:

      tophat2.0.0 -o thout -r 180 genome2 seq1 seq2

      It was ok.

      Comment

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