who knows how to figure out unigene from sequence ? Is there some bioinformatic tools can help to produce? 
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If you are asking about how to create a non-redundant set of sequences then have a look at the suggestion in this thread: http://seqanswers.com/forums/showthread.php?t=22909
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Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
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