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I am extremely new to Biopython and was hoping someone could help me. I have downloaded the complete GenBank files for Salmonella enterica serovar Typhimurium SL1344 (it has 3 plasmids and one chromosome and I have concatenated all of them into one file). I have a list of 123 gene names that I would like to make a Multi-FASTA file for. Is there an easy way to do this? Thank you so much for any and all help.
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I suggest you read http://www2.warwick.ac.uk/fac/sci/mo...genbank2fasta/ but also why not download the matching per-gene FASTA file from the NCBI and filter that?
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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