Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Download DNA sequences for RepeatMasker track

    1. I want to download the DNA sequences for the mouse mm9 RepeatMasker track from the UCSC Genome Browser. When I tried the Table Browser it got to 167 MB after ~10 minutes then stopped, having only completed chromosome 1 and part of 2. The file I downloaded ended with:

    Code:
    procedures have exceeded timeout: 1200 seconds, function has ended.
    My internet connection is very fast but it was downloading pretty slowly so I assume it's being limited by UCSC's speed. Should I be able to download this from the Table Browser or is there a better way?

    2. Here is what one sequence looks like:

    Code:
    >mm9_rmsk_L1_Mur2 range=chr1:3000002-3000156 5'pad=0 3'pad=0 strand=- repeatMasking=none
    AAATGTTAAATCTAAAAAAATCCTAACAAGAAACAGCCAGGAAATCTGGG
    ACACTATGAAAAGACCAAACCTAAGAAAAATAGGAATAAAAGAAGGACAA
    AAGTTTCAGCTGAAACACCCAGAAAACATATTAAACTAAATCATAGAAAA
    GAATT
    The header includes the repeat name (L1_Mur2), but I would also like the repeat Family and Class which you can get if you download the RepeatMasker track itself, but not if you download the actual sequences like I'm trying to do. I'm pretty sure I could use Perl and add correct Family and Class info to each sequence but if there is some way to get the sequences with this information already included it would save a bit of time.

    [Edit] Problem solved using bedtools getfasta with the genome fasta file and a bed file for each type of repeat.
    Last edited by biznatch; 03-24-2014, 01:19 PM.

Latest Articles

Collapse

  • seqadmin
    Best Practices for Single-Cell Sequencing Analysis
    by seqadmin



    While isolating and preparing single cells for sequencing was historically the bottleneck, recent technological advancements have shifted the challenge to data analysis. This highlights the rapidly evolving nature of single-cell sequencing. The inherent complexity of single-cell analysis has intensified with the surge in data volume and the incorporation of diverse and more complex datasets. This article explores the challenges in analysis, examines common pitfalls, offers...
    Today, 07:15 AM
  • seqadmin
    Latest Developments in Precision Medicine
    by seqadmin



    Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.

    Somatic Genomics
    “We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...
    05-24-2024, 01:16 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Today, 08:18 AM
0 responses
8 views
0 likes
Last Post seqadmin  
Started by seqadmin, Today, 08:04 AM
0 responses
10 views
0 likes
Last Post seqadmin  
Started by seqadmin, 06-03-2024, 06:55 AM
0 responses
13 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-30-2024, 03:16 PM
0 responses
27 views
0 likes
Last Post seqadmin  
Working...
X