Ngs

[Desai]

Dear Friends,

I am newbie in sequencing field. I have some questions in my mind.
(1) Why we prefer illumina more than 454 sequencing, when 454 gives
400bp reads length compare to ilumina which gives only 150 reads length?
(2) And a silly question, what does mean 2*150 or 1 * 250 ? I mean what is 1 or 2
stand for. Is is for single and pair?
Thank you in advance for answer or just view.

[lethalfang]

Dear Friends,

I am newbie in sequencing field. I have some questions in my mind.
(1) Why we prefer illumina more than 454 sequencing, when 454 gives
400bp reads length compare to ilumina which gives only 150 reads length?
(2) And a silly question, what does mean 2*150 or 1 * 250 ? I mean what is 1 or 2
stand for. Is is for single and pair?
Thank you in advance for answer or just view.

1) Direct comparison of Illumina and 454: http://www.plosone.org/article/info%...l.pone.0030087

2) I think it means paired end (2 x 150) vs. single read (1 x 150).

[AllSeq]

Dear Friends,

I am newbie in sequencing field. I have some questions in my mind.
(1) Why we prefer illumina more than 454 sequencing, when 454 gives
400bp reads length compare to ilumina which gives only 150 reads length?
(2) And a silly question, what does mean 2*150 or 1 * 250 ? I mean what is 1 or 2
stand for. Is is for single and pair?
Thank you in advance for answer or just view.

454 actually does a bit better than 400b reads - some users are able to produce close to 1000b reads. Even though Illumina has shorter reads (up to 2X300 on the MiSeq), it beat out 454 for one simple reason - price. Illumina's platforms generate data at a cost of about 1/100th what it would cost to generate on the 454. Roche simply didn't improve their system as fast as the other platforms (Illumina, Ion Torrent and even PacBio). As such, they've made the decision to shut down the platform in 2016.

You can learn more about the performance specs and costs of the various platforms on our NGS Knowledge Bank.

[Desai]

I got your point. I thought it related to quality means illumina gave better quality than Roche 454.

[AllSeq]

I got your point. I thought it related to quality means illumina gave better quality than Roche 454.

454 has decent quality, but it definitely has a homopolymer issue - strings of the same base are challenging for the detection system to get right, leading to indel errors. Ion Torrent suffers from the same issue (due to similar chemistries), but they've done more to correct it.