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  • phalaenopsis
    Junior Member
    • Jan 2010
    • 2

    #1

    samtools pileup GLF

    Hi,

    I work with multiple bam-files which I have

    1. merged (using samtools merge)
    2. sorted (using samtools sort)
    3. removed duplicates (using samtools rmdup)
    4. sorted (using samtools sort)

    I tried then to generate a GLF file
    (using samtools -g -f reference.fasta merged_file.bam > merged_file.glf).
    Samtools is running and generates GLF-files but before it stops, it throws out the following message
    '[bam_pileup_core] the input is not sorted (chromosomes out of order)'
    although the infile was sorted before (however, the bam-header flag was still set to 'unsorted')

    I am really confused as samtools does not abort the pileup and I get an output GLF-file (I just don't know whether it is right or not....)

    Thanks for your help!
    Last edited by phalaenopsis; 03-25-2010, 09:28 AM.

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