Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • Insane
    Junior Member
    • Oct 2009
    • 3

    #1

    SOAP and quality

    I am using SOAP to map my reads on the human genome.
    Since SOAP can work with .fasta or .fastq files, I thought it checks the quality when running.
    But I have the exact same results with my .fasta and my .fastq file, so I wonder if SOAP really cares about quality?
  • swbarnes2
    Senior Member
    • May 2008
    • 910

    #2
    Originally posted by Insane View Post
    I am using SOAP to map my reads on the human genome.
    Since SOAP can work with .fasta or .fastq files, I thought it checks the quality when running.
    But I have the exact same results with my .fasta and my .fastq file, so I wonder if SOAP really cares about quality?
    I don't think it makes a difference in the alignment at all. But when assessing bases that differ from the reference, it helps to know if the differing base is of high quality or not.

    Comment

    • Batalov
      Junior Member
      • Jan 2009
      • 2

      #3
      Originally posted by Insane View Post
      I am using SOAP to map my reads on the human genome.
      Since SOAP can work with .fasta or .fastq files, I thought it checks the quality when running.
      But I have the exact same results with my .fasta and my .fastq file, so I wonder if SOAP really cares about quality?
      Are you referring to SOAP2 or SOAP v.1?

      If v.1, then my impression was that it doesn't really deal with quality, only keeps it aligned to the sequences at the time of the report. Which is convenient.

      Comment

      • Insane
        Junior Member
        • Oct 2009
        • 3

        #4
        Thank you for your answers.
        I was refering to SOAP2 but maybe there is no difference between the 2 versions for this criteria...

        Comment

        Latest Articles

        Collapse

        • SEQadmin2
          Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
          by SEQadmin2



          Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

          Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
          ...
          10-14-2026, 09:55 AM
        • SEQadmin2
          New Genomics Technologies Take Aim at Long-Standing Limits
          by SEQadmin2


          Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

          We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
          ...
          09-28-2026, 10:25 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by SEQadmin2, 10-22-2026, 11:13 AM
        0 responses
        27 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 10-22-2026, 10:48 AM
        0 responses
        20 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 10-15-2026, 10:52 AM
        0 responses
        36 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 10-14-2026, 05:39 AM
        0 responses
        43 views
        0 reactions
        Last Post SEQadmin2  
        Working...