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  • vanillasky
    Member
    • Mar 2014
    • 42

    #1

    ORF Finder-Metagenome

    I've finished my first metagenome assembly and would like to know if there is a good ORF finder I could try out before proceeding to functional annotation with CAMERA for example.
  • maasha
    Senior Member
    • Apr 2009
    • 153

    #2
    Prodigal is really good. It's got a *meta* switch.

    Comment

    • vanillasky
      Member
      • Mar 2014
      • 42

      #3
      Thank you, I also found out CAMERA has an ORF finder as well.

      Comment

      • lac302
        Member
        • Dec 2012
        • 64

        #4
        vanillasky...are you mining assemblies for specific genes/enzymes?

        i'm trying to figure out a more automated way of picking out ORFs that relate to specific enzyme families.

        Comment

        • vanillasky
          Member
          • Mar 2014
          • 42

          #5
          Hi Lac302! Maybe you can clarify what you mean by mining assemblies? I am interested in annotating the contigs coming out of my assembled metagenomes. Ultimately, yes I would like to focus on those contigs containing genes related to a certain function.

          Comment

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