Hello everyone I received data from Illumina sequencing into qseq files from different LANE.
And barcode for different samples.
SO I tried to transform my qseq files into fastq and then use fastx_barcode_splitter.pl to demultiplex by the barcode tag, but I am retrieving less reads.
I want to know if there is another strategy to demultiplex.
Best Regards,
And barcode for different samples.
SO I tried to transform my qseq files into fastq and then use fastx_barcode_splitter.pl to demultiplex by the barcode tag, but I am retrieving less reads.
I want to know if there is another strategy to demultiplex.
Best Regards,
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