Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • milw
    Director NGS Services, Lucigen
    • Dec 2013
    • 12

    #1

    SSPACE and number of mate pairs utilized?

    hi, I've been using SSPACE with our internally made mate pair libraries to scaffold a set of E coli contigs. It seems that SSPACE is using only a few thousand of the mate pair reads to perform the scaffolding, and my question is whether that low number is related to the required linkage parameters? My mate pair libraries have a have 100,000 - 2 million paired reads, but that's probably overkill for the purpose of scaffolding 195 contigs?
    thx for any help/comments!
    -Scott
    Scott Monsma
    Sr Scientist at Lucigen
  • luc
    Senior Member
    • Dec 2010
    • 469

    #2
    The vast majority of your mates will not map to two different contigs.
    In addition SSPACE also only maps to the ends of the contigs that are "in-reach" of your mate-pair insert sizes (e.g. it maps reads only to the 2kb of each contig end).

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
      by SEQadmin2




      NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...
      10-30-2026, 06:46 AM
    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 10-28-2026, 09:57 AM
    0 responses
    45 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-28-2026, 09:51 AM
    0 responses
    26 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-22-2026, 11:13 AM
    0 responses
    74 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-22-2026, 10:48 AM
    0 responses
    48 views
    0 reactions
    Last Post SEQadmin2  
    Working...